Mitochondrial production of reactive oxygen species and incidence of age-associated lymphoma in OF1 mice: effect of alternate-day fasting

Mech Ageing Dev. 2005 Nov;126(11):1185-91. doi: 10.1016/j.mad.2005.06.007.


There is currently of a great interest investigating the role of nutrition in the prevention of age-associated disorders. The present study aimed to evaluate, on a particular strain of mice, the efficacy of alternate-day fasting on the mitochondrial production of free radical species and on the incidence of a specific cancer (lymphoma) in aged mice. Alternate fasting, that was initiated in middle age mice through a 4 month period, reduced significantly the incidence of lymphoma (0% versus 33% for controls). No remarkable difference was observed in the overall food consumption between alternate-fed (AF) and ad libitum (AL) mice, suggesting that the efficacy of alternate fasting did not really depend on calorie restriction. A significant decrease in the mitochondrial generation of reactive oxygen species (ROS) that was associated with a significant increase in spleen mitochondria SOD activity was observed when mice were maintained on intermittent fasting. Our results suggest that alternate fasting could exert a beneficial antioxidant effect and a modulation of the oxidative stress associated with aging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / physiology*
  • Animals
  • Catalase / metabolism
  • Eating
  • Fasting*
  • Female
  • Free Radicals / metabolism
  • Glutathione Peroxidase / metabolism
  • Humans
  • Lymphoma / metabolism*
  • Mice
  • Mice, Inbred Strains
  • Mitochondria / metabolism*
  • Reactive Oxygen Species / metabolism*
  • Spleen / enzymology
  • Superoxide Dismutase / metabolism


  • Free Radicals
  • Reactive Oxygen Species
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase