Several techniques are available in detecting variations in gene expression between different samples, such as SSH, RACE, etc. However, they can not be applied to analyze mitochondrial gene expression due to the specific characteristics of mitochondrial RNA. So some modifications were made to the conventional techniques. Here we reported a demonstration of this modified technique, taking rice mitochondria as materials. In this technique, using random hexamers to prime the RT, the resultant cDNA likely included coding regions because it was not locked to the poly(A) tail of the messenger RNA.