Multiparametric assay to screen and dissect the mode of action of anti-human immunodeficiency virus envelope drugs

Antimicrob Agents Chemother. 2005 Sep;49(9):3926-9. doi: 10.1128/AAC.49.9.3926-3929.2005.


A flow cytometry-based assay was used to simultaneously quantify X4 and R5 human immunodeficiency virus (HIV) envelope-mediated cell-to-cell viral transfer, cell death, and cell-to-cell fusion. In this assay, different anti-HIV envelope drugs showed characteristic inhibitory profiles for each measured parameter, allowing for the rapid identification of the mode of action of active compounds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-HIV Agents / pharmacology*
  • Cell Count
  • Cell Death / drug effects
  • Cell Fusion
  • Cell Line
  • Cell Survival / drug effects
  • Coculture Techniques
  • Drug Evaluation, Preclinical / methods*
  • HIV Infections / transmission*
  • HIV-1 / drug effects*
  • Humans
  • Tetrazolium Salts
  • Thiazoles
  • Viral Envelope Proteins / antagonists & inhibitors*
  • Viral Envelope Proteins / drug effects


  • Anti-HIV Agents
  • Tetrazolium Salts
  • Thiazoles
  • Viral Envelope Proteins
  • thiazolyl blue