Self-made frits for nanoscale columns in proteomics

Proteomics. 2005 Oct;5(15):3847-50. doi: 10.1002/pmic.200402010.

Abstract

We report here the production of self-made frits for nano-columns. The frits introduce a minor dead volume and can be placed in capillaries with a wide range of diameters (20-250 microm tested) in an extremely simple and low-cost procedure. The obtained columns appear to be comparable to "no-frit" columns with near-ideal chromatographic characteristics. We expect that this frit will be useful for the spotting of gradients onto MALDI plates but also where special ESI set-ups do not allow for "no-frit" solutions.

Publication types

  • Editorial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Liquid / instrumentation*
  • Chromosomal Proteins, Non-Histone
  • Mass Spectrometry / instrumentation
  • Nanotechnology / instrumentation*
  • Proteomics / instrumentation
  • Reproducibility of Results*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Transcription Factors

Substances

  • Chromosomal Proteins, Non-Histone
  • Transcription Factors