Protective effects of caffeic acid phenethyl ester (CAPE) on amikacin-induced nephrotoxicity in rats

Cell Biochem Funct. Jul-Aug 2006;24(4):363-7. doi: 10.1002/cbf.1264.


Amikacin (AK) has nephrotoxic side effects. AK-induced nephrotoxicity may be the consequence of oxidative stress and so anti-oxidant agents could be useful in reducing AK toxicity. Caffeic acid phenethyl ester (CAPE) was recently shown to have free radical scavenging ability and it reduces lipid peroxidation. For this purpose, the rats were distributed into three groups: (I) injected with vehicle (control); (II) injected (i.p.) with 1.2 g kg(-1) AK at a single dose; (III) injected (i.p.) with AK plus 10 micromol kg(-1) CAPE. Renal morphology was investigated by light microscopy. Tissue samples and trunk blood were also obtained to determine renal malondialdehyde (MDA), blood urea nitrogen (BUN) and creatinine (Cr) levels. MDA production was found to be higher in rats given AK than among control rats. CAPE administration before AK injection caused a significant decrease in MDA production. Morphological tubule damage in rats given AK was severe in the renal cortex, whereas in rats given AK plus CAPE, no histological changes occurred. It is concluded that CAPE could be useful for reducing the nephrotoxic effects of AK.

Publication types

  • Comparative Study

MeSH terms

  • Amikacin
  • Animals
  • Caffeic Acids / pharmacology*
  • Catalase / metabolism
  • Creatinine / metabolism
  • Female
  • Free Radical Scavengers / pharmacology
  • Glutathione Peroxidase / metabolism
  • Kidney Diseases / chemically induced
  • Kidney Diseases / metabolism
  • Kidney Diseases / pathology
  • Kidney Diseases / prevention & control*
  • Kidney Function Tests
  • Lipid Peroxidation
  • Malondialdehyde / metabolism
  • Oxidative Stress
  • Phenylethyl Alcohol / analogs & derivatives*
  • Phenylethyl Alcohol / pharmacology
  • Protective Agents / pharmacology*
  • Rats
  • Rats, Wistar
  • Sensitivity and Specificity
  • Superoxide Dismutase / metabolism


  • Caffeic Acids
  • Free Radical Scavengers
  • Protective Agents
  • Malondialdehyde
  • Amikacin
  • Creatinine
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • caffeic acid phenethyl ester
  • Phenylethyl Alcohol