Immunomodulatory function of orally administered thymosin alpha1

J Zhejiang Univ Sci B. 2005 Sep;6(9):873-6. doi: 10.1631/jzus.2005.B0873.


Objective: To investigate the immunological function of a yeast expression system for thymosin alpha1 (Talpha1).

Methods: A constructed Talpha1 yeast expression system was used to investigate the immunological function of orally administered Talpha1. Dried yeast containing three different concentration of Talpha1 was fed to normal Balb/c mice and other Balb/c mice whose immunities were inhibited in advance by cyclophosphamide. Synthesized Talpha1 peptide was used as positive control and dried yeast with empty plasmid was used as negative control. CD4(+) and CD8(+) levels were detected by flow cytometry assay. TNF-alpha, IFN-gamma, IL-2, IL-6 and IL-10 levels were detected by liquid chip.

Results: In normal Balb/c mice or immune inhibition Balb/c mice, CD8(+) levels were significantly increased. Especially in immune inhibition Balb/c mice, CD8(+) levels in synthesized Talpha1 group (18.77%+/-4.72%), small dose group (13.48%+/-6.17%) and large dose group (22.74%+/-1.09%) were significantly higher than that in empty yeast control group (7.49%+/-2.14%).

Conclusion: Orally administered Talpha1 has its certain immunomodulatory function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Animals
  • CD4-Positive T-Lymphocytes / drug effects
  • CD8-Positive T-Lymphocytes / drug effects
  • Cyclophosphamide / toxicity
  • Cytokines / metabolism
  • Immunologic Deficiency Syndromes / chemically induced
  • Immunologic Deficiency Syndromes / drug therapy
  • Immunologic Deficiency Syndromes / immunology
  • Immunologic Factors / administration & dosage*
  • Immunologic Factors / genetics
  • Immunosuppressive Agents / toxicity
  • Mice
  • Mice, Inbred BALB C
  • Recombinant Proteins / administration & dosage
  • Recombinant Proteins / genetics
  • Saccharomyces cerevisiae / genetics
  • Thymalfasin
  • Thymosin / administration & dosage
  • Thymosin / analogs & derivatives*


  • Cytokines
  • Immunologic Factors
  • Immunosuppressive Agents
  • Recombinant Proteins
  • Thymosin
  • Cyclophosphamide
  • Thymalfasin