Controlled fabrication of a biological vascular substitute

Biomaterials. 2006 Mar;27(7):1088-94. doi: 10.1016/j.biomaterials.2005.07.048. Epub 2005 Aug 29.


Autologous and synthetic vessel grafts have been used as a vascular substitute for cardiovascular bypass procedures. However, these materials are limited by the availability of appropriate caliber autologous vessels, increased susceptibility to thrombosis and intimal hyperplasia following surgery. Electrospinning technology offers the potential for controlling composition, structure and mechanical properties of biomaterials. Vascular graft scaffolds have been fabricated using electrospun polymer blends of Type I collagen, elastin from ligamentum nuchae, and poly (d,l-lactide-co-glycolide). This study demonstrates improved electrospinning characteristics versus previous studies by increasing polymer concentration and adding PLGA to the polymer blend. Additionally, new in vitro biocompatibility and mechanical testing data is presented. The scaffolds possess tissue composition and mechanical properties similar to native vessels. The electrospun vessel matrix is biocompatible and does not elicit local or systemic toxic effects when implanted in vivo. This study demonstrates the promise of electrospinning as a fabrication process for a functional vascular graft for clinical use.

MeSH terms

  • Animals
  • Bioartificial Organs
  • Biocompatible Materials / chemistry
  • Blood Vessel Prosthesis*
  • Cattle
  • Cell Culture Techniques / methods
  • Cell Proliferation
  • Cell Survival
  • Cells, Cultured
  • Collagen / chemistry*
  • Elastin / chemistry*
  • Endothelial Cells / cytology*
  • Endothelial Cells / physiology
  • Glycolates / chemistry*
  • Guided Tissue Regeneration / methods*
  • Lactic Acid
  • Materials Testing
  • Myocytes, Smooth Muscle / cytology*
  • Myocytes, Smooth Muscle / physiology
  • Polyglycolic Acid
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Shear Strength
  • Tensile Strength
  • Tissue Engineering / methods


  • Biocompatible Materials
  • Glycolates
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • Collagen
  • Elastin