Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum

Eur J Clin Microbiol Infect Dis. 2005 Aug;24(8):549-53. doi: 10.1007/s10096-005-1378-7.

Abstract

The study presented here was conducted to evaluate the performance of a double-antigen sandwich ELISA to detect antibodies in human serum against the coronavirus associated with severe acute respiratory syndrome (SARS). A recombinant partial nucleocapsid protein of SARS-associated coronavirus was used as a serodiagnostic antigen in the ELISA. A total of 2892 clinical serum samples were tested with the ELISA kit, which positively identified 25 of 35 (71.4%) samples of patients with confirmed SARS infection, 286 of 407 (70%) samples of patients suspected of having SARS, 229 of 302 (75.8%) samples of convalescent SARS patients, and 0 of 544 samples obtained from healthcare workers; only 1 of 1604 clinical samples obtained from patients with other diseases demonstrated a weakly positive result. These results indicate that the double-antigen sandwich ELISA is an effective screening method for the serodiagnosis of SARS-associated coronavirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral / blood*
  • Enzyme-Linked Immunosorbent Assay*
  • Humans
  • Nucleocapsid Proteins / immunology*
  • Recombinant Proteins / analysis
  • Recombinant Proteins / immunology
  • SARS Virus / genetics
  • SARS Virus / immunology
  • SARS Virus / isolation & purification*
  • Severe Acute Respiratory Syndrome / diagnosis*
  • Severe Acute Respiratory Syndrome / epidemiology
  • Severe Acute Respiratory Syndrome / immunology

Substances

  • Antibodies, Viral
  • Nucleocapsid Proteins
  • Recombinant Proteins