DXS6797 contains two STRs which can be easily haplotyped in both sexes

Int J Legal Med. 2006 Mar;120(2):61-6. doi: 10.1007/s00414-005-0003-8. Epub 2005 Aug 25.

Abstract

DXS6797 is a complex X-chromosomal locus which contains two variable short tandem repeats (STRs) (motif ATCT) separated by 128 non-polymorphic nucleotides. The two STRs can be cleaved apart by Taq I digestion. Conventionally, DXS6797 is typed by measuring the overall amplicon length, providing only eight alleles [polymorphism information content (PIC) 0.733, mean exclusion chance (MEC) 0.712]. Separate amplification would increase the discrimination but obscure the haplotype constellation in females. Therefore, we proceed by amplifying the whole sequence containing both repeats (DXS6797 I and DXS6797 II) using a Fam-labelled forward primer and a Tamra-labelled reverse primer. We then measure the length of the entire double-labelled amplicon and a Taq-I-digested aliquot to infer, for both males and females, compound haplotypes consisting of DXS6797 I and DXS6797 II repeat length. This procedure has the potential to provide 42 DXS6797 haplotypes. If the crossover rate between both STRs is assumed to be <1.5x10(-6), DXS6797 haplotypes could be used for kinship testing like STR alleles. In our German sample (780 X chromosomes), we determined 27 haplotypes (PIC 0.842, MEC 0,834) and in 220 meioses, we found no new mutations.

MeSH terms

  • Adolescent
  • Adult
  • Chromosomes, Human, X
  • DNA Fingerprinting / methods*
  • DNA Primers
  • Female
  • Gene Frequency
  • Genetic Linkage
  • Genetics, Population
  • Germany
  • Haplotypes*
  • Humans
  • Male
  • Middle Aged
  • Polymerase Chain Reaction
  • Tandem Repeat Sequences*
  • Taq Polymerase / genetics

Substances

  • DNA Primers
  • Taq Polymerase