The effect of the flavonoid diosmin, grape seed extract and red wine on the pulmonary metastatic B16F10 melanoma

Histol Histopathol. 2005 Oct;20(4):1121-9. doi: 10.14670/HH-20.1121.


Objective: To study the effect of different phenolic compounds and red wine on pulmonary metastatic melanoma.

Methods: Swiss mice were inoculated with 500000 melanocytes B16F10 and given oral doses of diosmin, grape seed extract (GSE) and red wine. A macroscopic count was made of the metastatic nodules on the lung surface and a microscopic study by image analysis of five sections, calculating the implantation percentage and tumoral growth and invasion indices.

Results: Macroscopically, the group treated with diosmin showed the greatest reduction (52%) in the number of metastatic nodules compared with the control group, which was treated with ethanol, while GSE and red wine caused decreases of 26.07 and 28.81%, respectively. Microscopically, there was a decrease in the implantation percentage after the administration of diosmin (79.4%) and red wine (20.19%), and an increase of 2.12% after the administration of GSE, all relative to the ethanol-treated control. As regards the growth index, diosmin produced a reduction of 67.44% and red wine a reduction of 20.62%, while GSE again produced an increase (25.33%). The reductions in the invasion index were 45.23, 31.65 and 17.57% with diosmin, GSE and red wine, respectively.

Conclusions: Diosmin originated the greatest reduction in pulmonary metastases, both at the macroscopic and microscopic levels.

Publication types

  • Comparative Study

MeSH terms

  • Administration, Oral
  • Animals
  • Diosmin / administration & dosage
  • Diosmin / pharmacology*
  • Female
  • Lung Neoplasms / prevention & control
  • Lung Neoplasms / secondary*
  • Melanocytes / pathology
  • Melanocytes / transplantation
  • Melanoma, Experimental / drug therapy*
  • Melanoma, Experimental / pathology
  • Mice
  • Neoplasm Invasiveness / pathology
  • Neoplasm Transplantation
  • Phytotherapy* / methods
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology*
  • Seeds
  • Vitis*
  • Wine*


  • Plant Extracts
  • Diosmin