Rhodopsin-EGFP knock-ins for imaging quantal gene alterations

Vision Res. 2005 Dec;45(28):3445-53. doi: 10.1016/j.visres.2005.07.016. Epub 2005 Aug 31.


We have developed an imaging approach to monitor changes in gene structure in photoreceptors. We review here, the strategy and recent progress. Knock-in mice bearing a human rhodopsin-EGFP fusion gene potentially allow detection of a single molecular event: correction of a single copy of a gene within an entire retina. These mice can also be used for imaging rhodopsin distribution, membrane structure, and trafficking in normal mice or in disease states, using confocal or multiphoton fluorescence imaging techniques. They represent tools for studying molecular triggers of photoreceptor development, for following stem cell populations, and for evaluating retinal transplantation experiments.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Feasibility Studies
  • Genetic Therapy / methods*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Mice
  • Photoreceptor Cells / metabolism*
  • Retina / pathology*
  • Retinal Degeneration / genetics*
  • Retinal Degeneration / pathology
  • Rhodopsin / genetics*


  • Green Fluorescent Proteins
  • Rhodopsin