DNA Vaccine Against the Non-Structural 1 Protein (NS1) of Dengue 2 Virus

Vaccine. 2006 May 22;24(21):4562-4. doi: 10.1016/j.vaccine.2005.08.022. Epub 2005 Aug 19.

Abstract

Dengue is one of the most important mosquito-borne viral disease causing dengue fever and/or dengue shock syndrome/haemorrhagic fever. In some reports, the non-structural protein 1 (NS1) has been identified as a promising antigen for the development of vaccines against dengue virus (DENV). Apparently, it can elicit a protective antibody response with complement-fixing activities. In order to investigate the potential of a DNA vaccine based on the NS1 protein against DENV, we used the plasmid pcTPANS1, which contains the secretory signal sequence derived from human tissue plasminogen activator (t-PA) fused to the full length of the DENV-2 NS1 gene. All Balb/c mice intramuscularly inoculated with the pcTPANS1 presented high levels of NS1-specifc antibodies. Vaccinated animals were challenged with intracerebral DENV-2 virus inoculations and a 100% survival was observed. In general, results demonstrate that the pcTPANS1 plasmid is able to induce protection in mice, and then may be used as a vaccination approach against DENV in further assays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / analysis
  • Dengue Virus / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Mice
  • Vaccines, DNA / immunology*
  • Viral Nonstructural Proteins / immunology*

Substances

  • Antibodies, Viral
  • NS1 protein, Dengue virus type 2
  • Vaccines, DNA
  • Viral Nonstructural Proteins