Room-air resuscitation causes less damage to heart and kidney than 100% oxygen

Am J Respir Crit Care Med. 2005 Dec 1;172(11):1393-8. doi: 10.1164/rccm.200412-1740OC. Epub 2005 Sep 1.


Rationale: Pure oxygen causes more oxidative stress than room air in resuscitation of asphyctic neonates, and consequently could be associated with increased tissue damage.

Objectives: To compare damage caused to heart and kidneys on reoxygenation in severely asphyctic term neonates resuscitated with room air (RAR) or 100% oxygen (OxR). Nonasphyctic term newborn infants served as a control group.

Methods and measurements: This is a prospective randomized clinical trial masked for the gas mixture. Reduced glutathione (GSH), oxidized glutathione (GSSG), and superoxide dismutase (SOD) activity were measured to assess oxidative stress. Plasma cardiac troponin T (cTnT) and urinary N-acetyl-glucosaminidase (NAG) assessed cardiac and renal damage, respectively. Daily determinations of NAG for a 2-wk period were performed to monitor postasphyctic renal damage.

Main results: Both asphyctic groups showed oxidative stress when compared with the control group as evidenced by diminished GSH/GSSG ratios, adaptive increases in SOD activity, and higher values of NAG and cTnT (markers of tissue damage). However, the OxR group showed significantly higher values of NAG and cTnT, lower GSH/GSSG ratios, and higher SOD activity than the RAR group. Moreover, NAG values persisted in being higher than normal in the OxR group for 2 wk after birth, whereas NAG in the RAR group dropped to normal within the first week. A linear correlation between cTnT or NAG and GSSG was found.

Conclusions: The use of room air on resuscitation causes less oxidative stress and damage to heart and kidney than pure oxygen.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosaminidase / urine
  • Air
  • Asphyxia Neonatorum / blood
  • Asphyxia Neonatorum / therapy*
  • Asphyxia Neonatorum / urine
  • Biomarkers / blood
  • Biomarkers / urine
  • Cardiomyopathies / prevention & control*
  • Chromatography, High Pressure Liquid
  • Creatinine / blood
  • Creatinine / urine
  • Follow-Up Studies
  • Humans
  • Infant, Newborn
  • Kidney Diseases / prevention & control*
  • Oxidative Stress / physiology
  • Oxygen Inhalation Therapy*
  • Prospective Studies
  • Respiratory Therapy / methods*
  • Resuscitation / methods*
  • Severity of Illness Index
  • Superoxide Dismutase / blood
  • Treatment Outcome
  • Troponin T / blood


  • Biomarkers
  • Troponin T
  • Creatinine
  • Superoxide Dismutase
  • Acetylglucosaminidase