Abstract
The extract of Anemarrhenae Rhizoma (rhizomes of Anemarrhena asphodeloides BUNGE) showed neurotrophic activity toward rat pheochromocytoma (PC-12) cells. Bioassay-guided purification afforded four compounds, 2,6,4'-trihydroxy-4-methoxybenzophenone (1), 7-hydroxy-3-(4-hydroxybenzyl)chroman (2), broussonin B (3), and cis-hinokiresinol (4). Compounds 1-3 induced neurite outgrowth in PC-12 cells at concentration of 50 microg/ml, while 4 was less active. In addition, compounds 2-4 showed moderate inhibitory activities against a chymotrypsin-like activity of the proteasome.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkanes / pharmacology*
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Anemarrhena / chemistry*
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Animals
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Benzyl Compounds / pharmacology*
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Central Nervous System Agents / pharmacology*
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Chromans / pharmacology*
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Chymotrypsin / chemistry
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Magnetic Resonance Spectroscopy
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Nervous System / drug effects*
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Neurites / drug effects
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PC12 Cells
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Phenols / pharmacology*
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Plant Roots / chemistry
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Protease Inhibitors / chemistry
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Protease Inhibitors / isolation & purification
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Protease Inhibitors / pharmacology*
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Rats
Substances
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7-hydroxy-3-(4-hydroxybenzyl)chroman
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Alkanes
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Benzyl Compounds
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Central Nervous System Agents
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Chromans
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Phenols
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Protease Inhibitors
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broussonin B
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Chymotrypsin