Epicutaneous antigen exposure primes for experimental eosinophilic esophagitis in mice

Gastroenterology. 2005 Sep;129(3):985-94. doi: 10.1053/j.gastro.2005.06.027.


Background & aims: Eosinophilic esophagitis (EE) is frequently associated with atopic disease, including dermatitis and asthma. Data are emerging that atopic skin may provide an early entry point for antigen sensitization. We aimed to test the hypothesis that epicutaneous exposure to antigen primes for subsequent respiratory antigen-induced EE.

Methods: Wild-type and genetically engineered mice were subjected to epicutaneous antigen sensitization and the development of experimental EE, and immune responses were examined.

Results: We show that exposure to antigen via the epicutaneous route primes for marked eosinophilic inflammation in the esophagus triggered by a single airway antigen challenge. The development of experimental EE is associated with significant skin eosinophilia, accelerated bone marrow eosinophilopoiesis, blood eosinophilia, and large increases in serum antigen-specific immunoglobulin G1/immunoglobulin E using ovalbumin or Aspergillus fumigatus as the epicutaneous antigen. Mechanistic analysis with gene-targeted mice showed that interleukin-5 was required for esophageal eosinophilia and that interleukin-4, interleukin-13, and STAT6 contributed to a lesser extent.

Conclusions: These findings provide the first evidence that epicutaneous exposure to allergens potently primes for EE via a Th2-dependent mechanism.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Allergens / immunology
  • Animals
  • Antigens / immunology*
  • Cytokines / blood
  • Disease Models, Animal
  • Eosinophilia / immunology*
  • Eosinophils
  • Esophagitis / immunology*
  • Genetic Engineering
  • Immunization
  • Immunoglobulin E / blood
  • Immunoglobulin E / immunology
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology
  • Leukocyte Count
  • Mice
  • Ovalbumin / immunology
  • Th2 Cells / immunology


  • Allergens
  • Antigens
  • Cytokines
  • Immunoglobulin G
  • Immunoglobulin E
  • Ovalbumin