LPS induces stefin A3 expression in mouse primary cultured glial cells

Brain Res Mol Brain Res. 2005 Oct 31;140(1-2):138-41. doi: 10.1016/j.molbrainres.2005.07.007. Epub 2005 Sep 6.


We searched for a gene that is up-regulated in response to LPS at a later time point in primary cultured glial cells. Using a Gene Chip Probe Array, we identified stefin A3, which is known as a cysteine protease inhibitor. As assessed by RT-PCR, we observed a time-dependent (2 to 48 h) up-regulation of stefin A3. The results indicate that stefin A3 is involved in infection and inflammation at a later time point.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cystatin A
  • Cystatin B
  • Cystatins / genetics*
  • DNA Primers
  • Gene Expression Regulation / drug effects*
  • Lipopolysaccharides / pharmacology*
  • Mice
  • Mice, Inbred C57BL
  • Neuroglia / cytology
  • Neuroglia / physiology*
  • Oligonucleotide Array Sequence Analysis


  • Cstb protein, mouse
  • Cystatin A
  • Cystatins
  • DNA Primers
  • Lipopolysaccharides
  • Stfa3 protein, mouse
  • Cystatin B