Coding and noncoding genomic regions of Entamoeba histolytica have significantly different rates of sequence polymorphisms: implications for epidemiological studies

J Clin Microbiol. 2005 Sep;43(9):4815-9. doi: 10.1128/JCM.43.9.4815-4819.2005.

Abstract

To evaluate genetic variability among Entamoeba histolytica strains, we sequenced 9,077 bp from each of 14 isolates. The polymorphism rates from coding and noncoding regions were significantly different (0.07% and 0.37%, respectively), indicating that these regions are subject to different selection pressures. Additionally, single nucleotide polymorphisms (SNPs) potentially associated with specific clinical outcomes were identified.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Entamoeba histolytica / classification*
  • Entamoeba histolytica / genetics
  • Entamoebiasis / epidemiology*
  • Entamoebiasis / parasitology
  • Epidemiologic Studies
  • Female
  • Genetic Variation
  • Genome, Protozoan
  • Genotype
  • Humans
  • Male
  • Molecular Sequence Data
  • Open Reading Frames / genetics*
  • Polymorphism, Genetic
  • Polymorphism, Single Nucleotide / genetics*
  • RNA, Untranslated / genetics*
  • Sequence Analysis, DNA

Substances

  • RNA, Untranslated

Associated data

  • GENBANK/AY956427
  • GENBANK/AY956428
  • GENBANK/AY956429
  • GENBANK/AY956430
  • GENBANK/AY956431
  • GENBANK/AY956432
  • GENBANK/AY956433
  • GENBANK/AY956434
  • GENBANK/AY956435
  • GENBANK/AY956436
  • GENBANK/AY956437
  • GENBANK/AY956438
  • GENBANK/AY956439
  • GENBANK/AY956440