Evaluation of the effect of incorporation of dibutyryl cyclic adenosine monophosphate in an in situ-forming hydrogel wound dressing based on oxidized alginate and gelatin

Biomaterials. 2006 Mar;27(8):1355-61. doi: 10.1016/j.biomaterials.2005.08.021. Epub 2005 Sep 16.


Cyclic adenosine monophosphate (cAMP) has long been regarded as a second messenger and a regulator of human keratinocyte proliferation. To explore more effective wound management, dibutyryl cyclic adenosine monophosphate (DBcAMP), a lipophilic analog of cAMP was incorporated into an in situ-forming hydrogel wound dressing based on periodate-oxidized alginate and gelatin. In vitro release of DBcAMP from the matrix into phosphate buffered saline was slow and increased with time. Only 50-60% of the compound was released into the medium over a period of 2 days suggestive of a sustained release into the wound bed over a period of few days. The wound-healing efficacy of the DBcAMP-incorporated dressing was evaluated on experimental full-thickness wounds in a rat model. It was found that dressing promoted wound healing leading to complete re-epithelialization of wounds within 10 days, whereas control wounds took 15 days for complete re-epithelialization. Data obtained in this study showed that the presence of DBcAMP accelerated healing and re-epithelialization of full-thickness wounds.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / metabolism*
  • Animals
  • Biocompatible Materials*
  • Biological Dressings*
  • Bucladesine*
  • Drug Evaluation, Preclinical
  • Gelatin / metabolism*
  • Glucuronic Acid / metabolism
  • Hexuronic Acids / metabolism
  • Hydrogels*
  • Male
  • Oxidation-Reduction
  • Rats
  • Rats, Wistar
  • Skin / injuries
  • Skin / pathology
  • Time Factors
  • Wound Healing / physiology*


  • Alginates
  • Biocompatible Materials
  • Hexuronic Acids
  • Hydrogels
  • Bucladesine
  • Glucuronic Acid
  • Gelatin