Human THROMBOSPONDIN-1 gene contains a natural antisense transcript, and characterization of its expression in human multiple tissues and cells

DNA Seq. 2005 Aug;16(4):295-9. doi: 10.1080/10425170500213654.

Abstract

Human THROMBOSPONDIN-1 play versatile roles in platelet aggregation, angiogenesis, and tumorigenesis, which forms a disulfide-linked homotrimeric complex. Here we reported that the genomic lotus of TSP1 also transcribes a natural antisense transcript (NAT) with an overlapping and reverse complement region against TSP1. The NAT of TSP1 was expressed in human testis, lung, thymus, colon, placenta, kidney and skeleton muscle, revealed by PCR amplification. It was also expressed in some tumor cell lines. The identification of NAT of TSP1 would be of significant importance to understand the functions of TSP1, and it would also suggest the potential attempt of using RNA interference for related tumor therapy, for instance, breast cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Line, Tumor
  • DNA, Antisense / genetics*
  • DNA, Antisense / metabolism
  • Gene Expression
  • Humans
  • Molecular Sequence Data
  • Species Specificity*
  • Thrombospondin 1 / genetics*
  • Thrombospondin 1 / metabolism
  • Tissue Distribution

Substances

  • DNA, Antisense
  • Thrombospondin 1