Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09)

Clin Diagn Lab Immunol. 2005 Sep;12(9):1127-9. doi: 10.1128/CDLI.12.9.1127-1129.2005.


Antibody-secreting cell (ASC) and antibodies in lymphocyte supernatant (ALS) assays are used to assess intestinal mucosal responses to enteric infections and vaccines. The ALS assay, performed on cell supernatants, may represent a convenient alternative to the more established ASC assay. The two methods, measuring immunoglobulin A to Salmonella enterica serovar Typhi lipopolysaccharide, were compared in volunteers vaccinated with a live-attenuated typhoid vaccine M01ZH09. The specificity of the ALS assay compared to the ASC assay was excellent (100%), as was sensitivity (82%). The ALS assay was less sensitive than the ASC assay at <or=42 spots/10(6) peripheral blood lymphocytes.

Publication types

  • Clinical Trial
  • Comparative Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Antibody-Producing Cells / immunology
  • Humans
  • Immunoglobulin A / immunology
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / immunology*
  • Lymphocytes / immunology
  • Salmonella typhi / immunology*
  • Typhoid Fever / immunology
  • Typhoid Fever / prevention & control*
  • Typhoid-Paratyphoid Vaccines / administration & dosage*
  • Typhoid-Paratyphoid Vaccines / immunology*


  • Immunoglobulin A
  • Typhoid-Paratyphoid Vaccines
  • typhoid vaccine M01ZH09