Abstract
The ets-1 proto-oncogene codes for a transcription factor. In order to understand how ets-1 is regulated, we have cloned its promoter. We show that the promoter is inducible by serum and expression of c-Fos and c-Jun, and it is positively auto-regulated by its gene product. A 50 base-pair sequence is sufficient to confer c-Fos + c-Jun and c-Ets-1 responsiveness to a heterologous promoter. This element contains two AP1 and one Ets-1 like motifs. Striking, AP-1 and Ets-1 motifs are found in oncogene responsive units (ORU's) of other promoters, suggesting that combining these motifs is a common mechanism for generating mitogen responsive transcription elements.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Blood
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Cell Line
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Consensus Sequence
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Culture Media
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Gene Expression Regulation*
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Humans
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Molecular Sequence Data
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Nuclear Proteins / metabolism
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Oligodeoxyribonucleotides / chemistry
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Promoter Regions, Genetic*
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Proto-Oncogene Mas
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Proto-Oncogene Protein c-ets-1
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins c-ets
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Proto-Oncogene Proteins c-jun / genetics*
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Proto-Oncogenes
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RNA, Messenger / genetics
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Regulatory Sequences, Nucleic Acid
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Transcription Factors*
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Transcription, Genetic
Substances
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Culture Media
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ETS1 protein, human
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MAS1 protein, human
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Nuclear Proteins
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Oligodeoxyribonucleotides
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Proto-Oncogene Mas
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Proto-Oncogene Protein c-ets-1
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-ets
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Proto-Oncogene Proteins c-jun
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RNA, Messenger
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Transcription Factors