Mobilization of recombinant plasmids from Staphylococcus aureus into coagulase negative Staphylococcus species

Plasmid. 1992 Mar;27(2):164-8. doi: 10.1016/0147-619x(92)90017-5.

Abstract

pC221, a small nonconjugative staphylococcal plasmid, can be mobilized between staphylococci by pG01, a larger conjugative plasmid. pC221 carries the two transacting genes, mobA and mobB, which are needed for its mobilization. The products of these genes create a site-specific single-stranded nick (mobA) and then facilitate DNA transfer (mobB). Several useful Escherichia coli-staphylococcal shuttle plasmids containing the cloned single-stranded nick site were created and successfully mobilized into Staphylococcus aureus and two coagulase-negative staphylococci, S. epidermidis and S. saprophyticus, by providing mob genes (pC221) and conjugative transfer genes (pG01) in trans in the donor. These vectors may offer a genetic system for the introduction of recombinant plasmids into coagulase negative staphylococci.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins*
  • Coagulase / metabolism
  • Conjugation, Genetic / genetics*
  • DNA, Recombinant / genetics*
  • DNA-Binding Proteins / genetics
  • Drug Resistance, Microbial / genetics
  • Plasmids / genetics*
  • Staphylococcus aureus / enzymology
  • Staphylococcus aureus / genetics*
  • Staphylococcus epidermidis / enzymology
  • Staphylococcus epidermidis / genetics
  • Trans-Activators*

Substances

  • Bacterial Proteins
  • Coagulase
  • DNA, Recombinant
  • DNA-Binding Proteins
  • RSF1010 MobB protein, Staphylococcus
  • Trans-Activators