Basic molecular biology

Paediatr Respir Rev. 2005 Sep;6(3):199-208. doi: 10.1016/j.prrv.2005.06.006.

Abstract

Rapid advances in molecular biology over the past 20 years have and will continue to impact on the practice of medicine. Advances in molecular biology are having an immense impact in determining the underlying aetiology of lung disease and its treatment. In this review, basic molecular biology techniques will be discussed with examples of how these techniques are used in clinical practice.

MeSH terms

  • Blotting, Northern
  • Blotting, Western
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Gene Expression / physiology
  • Humans
  • Immunohistochemistry
  • Molecular Biology*
  • Polymerase Chain Reaction
  • Protein C / metabolism
  • RNA, Messenger / physiology
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Protein C
  • RNA, Messenger