Identification of nitrated proteins in the normal rat brain using a proteomics approach

Neurol Res. 2005 Sep;27(6):630-3. doi: 10.1179/016164105X22039.

Abstract

Background: The nitration of tyrosine has been suggested to play a role in the pathogenesis of neurodegenerative disorders such as amyotrophic lateral sclerosis (ALS), Parkinson's disease (PD) and Alzheimer's disease (AD).

Methods: In the present study, we identified four targets of protein nitration, T-complex polypeptide 1 alpha subunit (TCP-1), neurofilament L (NFL), glial fibrillary acidic protein (GFAP) and clathrin heavy chain (CHC), in the normal rat cortex using a proteomics approach.

Conclusions: There have been no reports on these proteins being identified by proteomics as nitrated forms in the brain. For further study, we have to investigate alterations in these nitrated proteins during aging and in neurodegenerative disorders.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • Brain Chemistry*
  • Cerebral Cortex / metabolism*
  • Chaperonin Containing TCP-1
  • Chaperonins / isolation & purification
  • Chaperonins / metabolism
  • Chromatography, High Pressure Liquid / methods
  • Clathrin Heavy Chains / isolation & purification
  • Clathrin Heavy Chains / metabolism
  • Electrophoresis, Gel, Two-Dimensional / methods
  • Glial Fibrillary Acidic Protein / isolation & purification
  • Glial Fibrillary Acidic Protein / metabolism
  • Male
  • Neurofilament Proteins / isolation & purification
  • Neurofilament Proteins / metabolism
  • Nitrates / metabolism*
  • Proteomics*
  • Rats
  • Rats, Wistar

Substances

  • Glial Fibrillary Acidic Protein
  • Neurofilament Proteins
  • Nitrates
  • neurofilament protein L
  • Clathrin Heavy Chains
  • Chaperonin Containing TCP-1
  • Chaperonins