An improved arbitrary primed PCR method for rapid characterization of transposon insertion sites

J Microbiol Methods. 2005 Oct;63(1):89-94. doi: 10.1016/j.mimet.2005.02.011. Epub 2005 Mar 19.

Abstract

Modifications were made to published arbitrary primed polymerase chain reaction (AP-PCR) procedures that resulted in increased specificity and sensitivity. Several arbitrary primer sequences were also evaluated, resulting in recommendations for primer design.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA Primers
  • DNA Transposable Elements / genetics*
  • Polymerase Chain Reaction / methods*
  • Streptococcus sanguis / genetics

Substances

  • DNA Primers
  • DNA Transposable Elements