Rapid identification of Lactobacillus acidophilus by restriction analysis of the 16S-23S rRNA intergenic spacer region and flanking 23S rRNA gene

Biotechnol Lett. 2005 Aug;27(16):1183-8. doi: 10.1007/s10529-005-0014-z.


A rapid molecular approach was developed for the initial identification of Lactobacillus acidophilus strains which are difficult to identify using a single biochemical test. The 16S-23S rRNA intergenic spacer regions and flanking 23S rRNA genes of 19 strains of lactobacilli were amplified and the nucleotide sequences and restriction site polymorphisms were analyzed. AluI was the most useful of the restriction enzymes analyzed and produced reproducible digestion profiles in the L. helveticus, L. plantarum, and L. casei groups, as well as in L. acidophilus. This restriction fragment length polymorphism method may be useful for the identification of L. acidophilus strains in dairy products.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Flanking Region / genetics
  • Colony Count, Microbial / methods*
  • DNA, Intergenic / genetics
  • Lactobacillus acidophilus / genetics*
  • Lactobacillus acidophilus / isolation & purification*
  • RNA, Ribosomal, 16S / genetics*
  • RNA, Ribosomal, 23S / genetics*
  • Restriction Mapping / methods*
  • Sequence Analysis, RNA / methods*


  • DNA, Intergenic
  • RNA, Ribosomal, 16S
  • RNA, Ribosomal, 23S