Inhibition of human UDP-glucose dehydrogenase expression using siRNA expression vector in breast cancer cells

Biotechnol Lett. 2005 Aug;27(16):1229-32. doi: 10.1007/s10529-005-0022-z.

Abstract

UDP-glucose dehydrogenase (UGDH) catalyzes two oxidations of UDP-glucose to yield UDP-glucuronic acid. Pathological over-production of extracellular matrix components may be linked to the availability of UDP-glucuronic acid, therefore UGDH is a potential therapeutic target. RNA interference (RNAi) has been adapted to knock down the expression of human UGDH. A UGDH siRNA plasmid was constructed using a pRNA-U6.1/Neo vector and transfected into breast cancer cells, ZR-75-1, with an efficiency of up to 50%. Western blot analysis showed that the UGDH expression was efficiently knocked down at protein levels by RNAi in ZR-75-1 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / enzymology*
  • Breast Neoplasms / genetics*
  • Cell Line, Tumor
  • Gene Expression Regulation, Enzymologic / genetics*
  • Gene Expression Regulation, Neoplastic / genetics*
  • Gene Silencing
  • Genetic Vectors / genetics
  • Humans
  • RNA, Small Interfering / genetics*
  • Transfection / methods*
  • Uridine Diphosphate Glucose Dehydrogenase / genetics*
  • Uridine Diphosphate Glucose Dehydrogenase / metabolism*

Substances

  • RNA, Small Interfering
  • Uridine Diphosphate Glucose Dehydrogenase