Histological assessment in grafts of highly purified beta-tricalcium phosphate (OSferion) in human bones

Biomaterials. 2006 Mar;27(8):1542-9. doi: 10.1016/j.biomaterials.2005.08.034. Epub 2005 Sep 13.


Prominent osteoconductive activity and the biodegradable nature of commercially available beta-tricalcium phosphate (beta-TCP, OSferion) have been documented in animal experiments. We analyzed four cases of involving grafted OSferion in human bone with respect to histological features by routine hematoxylin and eosin staining, silver impregnation, immunohistochemistry and in situ hybridization. OSferion affords early bioresorption by osteoclasts, vascular invasion of macropores and osteoblastic cell attachment on the surface on the ceramic surface 14 days after grafting. Prominent bone formation and direct bone connection between preexisting bone and OSferion were evident 28 days after grafting. Nearly the entire TCP surface was covered by lamellar bone; additionally, active osteoblastic lining and attachment of the osteoclast-like giant cells were not observed 72 weeks after grafting. Silver impregnation revealed the presence of collagen fibrils within probable micropores of OSferion.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Biocompatible Materials* / isolation & purification
  • Biocompatible Materials* / therapeutic use
  • Bone Substitutes* / isolation & purification
  • Bone Substitutes* / therapeutic use
  • Bone and Bones / cytology*
  • Bone and Bones / pathology
  • Calcium Phosphates* / isolation & purification
  • Calcium Phosphates* / therapeutic use
  • Durapatite
  • Female
  • Femoral Fractures / pathology
  • Femoral Fractures / surgery
  • Hip Fractures / pathology
  • Hip Fractures / surgery
  • Humans
  • Male
  • Middle Aged
  • Osteosarcoma / pathology
  • Osteosarcoma / surgery


  • Biocompatible Materials
  • Bone Substitutes
  • Calcium Phosphates
  • beta-tricalcium phosphate
  • Durapatite