Cell-type specific regulation of serotonergic identity by the C. elegans LIM-homeodomain factor LIM-4

Dev Biol. 2005 Oct 15;286(2):618-28. doi: 10.1016/j.ydbio.2005.08.013. Epub 2005 Sep 13.

Abstract

How a common neurotransmitter phenotype specified in neurons of different origins is an outstanding issue in neuronal development and function. In C. elegans larvae, serotonin is synthesized in 2 pairs of neurons, the secretory neurons NSM and the chemosensory neurons ADF. In order to delineate the molecular mechanisms of serotonergic phenotype establishment, we have screened for neuron-specific serotonin deficient (nss) mutants. Our prior study showed that the POU-homeodomain factor UNC-86 is expressed in and required for the NSM neurons to adopt serotonergic phenotype and correct pathfinding, whereas ADF are unaffected in unc-86-null mutants. Here, we report that the LIM-homeodomain factor LIM-4 regulates ADF serotonergic phenotype. In lim-4 mutants, many aspects of ADF differentiation occur, however, they fail to express serotonin phenotype and exhibit aberrant cilia properties. LIM-4 expression rises in the neuroblast that produces two distinct neurons: ADF and the olfactory neuron AWB. We show that lim-4 is regulated by separable mechanisms to determine disparate subtype identities in these two neuronal types. In vivo promoter analyses reveal that cis-element(s) within introns are necessary and sufficient to direct lim-4 to specify serotonergic phenotype, whereas its 5'-upstream sequence directs lim-4 function in AWB. Thus, a transcription factor may act independently to specify distinct differentiation traits in two sister cells. We propose that serotonergic identity is specified in cell-specific contexts to coordinate the development and function.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins / biosynthesis
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / physiology*
  • Enhancer Elements, Genetic
  • Homeodomain Proteins / biosynthesis
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / physiology*
  • LIM-Homeodomain Proteins
  • Mutation
  • Neurons / cytology
  • Neurons / metabolism*
  • POU Domain Factors / biosynthesis
  • POU Domain Factors / physiology
  • Phenotype
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / physiology*
  • Serotonin / biosynthesis*
  • Serotonin / deficiency
  • Transcription Factors / genetics
  • Transcription Factors / physiology*

Substances

  • Caenorhabditis elegans Proteins
  • Homeodomain Proteins
  • LIM-Homeodomain Proteins
  • Lim-4 protein, C elegans
  • POU Domain Factors
  • Recombinant Fusion Proteins
  • Transcription Factors
  • unc-86 protein, C elegans
  • Serotonin