Evaluation of two TaqMan PCR assays for the detection of HIV-1 proviral DNA in blood samples

J Microbiol Methods. 2006 May;65(2):350-3. doi: 10.1016/j.mimet.2005.08.003. Epub 2005 Sep 16.


This study compared two published TaqMan PCR assays targeting different regions of the HIV-1 genome for detection of HIV-1 proviral DNA. The gag specific PCR demonstrated a lower sensitivity than the assay targeting the LTR region. The LTR assay is a highly reproducible and specific technique for HIV-1 proviral DNA detection.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • DNA, Viral / blood*
  • Genes, gag*
  • HIV Infections / diagnosis
  • HIV Infections / virology
  • HIV Long Terminal Repeat
  • HIV-1 / genetics
  • HIV-1 / isolation & purification*
  • Humans
  • Polymerase Chain Reaction / methods*
  • Proviruses / genetics
  • Proviruses / isolation & purification*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Taq Polymerase


  • DNA, Viral
  • Taq Polymerase