Wee1B is an oocyte-specific kinase involved in the control of meiotic arrest in the mouse

Curr Biol. 2005 Sep 20;15(18):1670-6. doi: 10.1016/j.cub.2005.07.056.

Abstract

In most species, the meiotic cell cycle is arrested at the transition between prophase and metaphase through unclear somatic signals. Activation of the Cdc2-kinase component of maturation promoting factor (MPF) triggers germinal vesicle breakdown after the luteinizing hormone (LH) surge and reentry into the meiotic cell cycle. Although high levels of cAMP and activation of protein kinase A (PKA) play a critical role in maintaining an inactive Cdc2, the steps downstream of PKA in the oocyte remain unknown. Using a small-pool expression-screening strategy, we have isolated several putative PKA substrates from a mouse oocyte cDNA library. One of these clones encodes a Wee1-like kinase that prevents progesterone-induced oocyte maturation when expressed in Xenopus oocytes. Unlike the widely expressed Wee1 and Myt1, mWee1B mRNA and its protein are expressed only in oocytes, and mRNA downregulation by RNAi injection in vitro or transgenic overexpression of RNAi in vivo causes a leaky meiotic arrest. Ser15 residue of mWee1B is the major PKA phosphorylation site in vitro, and the inhibitory effects of the kinase are enhanced when this residue is phosphorylated. Thus, mWee1B is a key MPF inhibitory kinase in mouse oocytes, functions downstream of PKA, and is required for maintaining meiotic arrest.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Cyclic AMP-Dependent Protein Kinases / genetics
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • Gene Library
  • Maturation-Promoting Factor / genetics
  • Maturation-Promoting Factor / metabolism*
  • Meiosis / genetics*
  • Mice
  • Molecular Sequence Data
  • Oocytes / metabolism*
  • Phosphorylation
  • Protein-Tyrosine Kinases / genetics
  • Protein-Tyrosine Kinases / metabolism*
  • RNA Interference
  • RNA, Messenger / metabolism*
  • Sequence Alignment
  • Sequence Analysis, DNA

Substances

  • Cell Cycle Proteins
  • RNA, Messenger
  • Wee1B protein, mouse
  • Protein-Tyrosine Kinases
  • Cyclic AMP-Dependent Protein Kinases
  • Maturation-Promoting Factor

Associated data

  • GENBANK/DQ011691