Abstract
Transgenic plants are a novel way to produce and deliver oral vaccines. Arabidopsis thaliana material shown previously to express the tuberculosis (TB) antigen ESAT-6 fused to the B subunit of Escherichia coli heat-labile enterotoxin (LTB) was fed to mice and the resulting immune response investigated. The plant-made LTB-ESAT-6 fusion protein induced antigen-specific responses from CD4+ cells and increased IFN-gamma production, indicating a Th1 response. In addition, a Th2 response was induced in the Peyer's patch. This is the first report of an orally delivered, subunit, tuberculosis vaccine priming an antigen-specific, Th1 response.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Administration, Oral
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Animals
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Antigens, Bacterial / biosynthesis
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Antigens, Bacterial / immunology
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Arabidopsis / genetics*
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Arabidopsis / metabolism*
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Bacterial Proteins
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Bacterial Toxins / biosynthesis
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Bacterial Toxins / immunology
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Cell Separation
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Enterotoxins / biosynthesis
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Enterotoxins / immunology
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Escherichia coli Proteins / biosynthesis
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Escherichia coli Proteins / immunology
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Female
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Flow Cytometry
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Immunity, Cellular / immunology
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Leukocytes / immunology
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Lung / cytology
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Lung / immunology
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Lymph Nodes / cytology
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Lymph Nodes / immunology
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Mice
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Mice, Inbred C57BL
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Peyer's Patches / immunology
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Peyer's Patches / metabolism
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Plants, Genetically Modified
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Spleen / cytology
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Spleen / immunology
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Th1 Cells / immunology
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Th2 Cells / immunology
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Tuberculosis Vaccines / administration & dosage
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Tuberculosis Vaccines / biosynthesis*
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Tuberculosis Vaccines / immunology*
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Vaccination
Substances
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Antigens, Bacterial
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Bacterial Proteins
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Bacterial Toxins
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ESAT-6 protein, Mycobacterium tuberculosis
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Enterotoxins
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Escherichia coli Proteins
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Tuberculosis Vaccines
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heat-labile enterotoxin, E coli