Lead is one of the industrially heavy metals that caused adverse effects on male reproductive system among battery factory workers, but information on the possible impact of lead on the structural integrity of sperm cell is limited. Thus present study was undertaken to assess the structural details of human spermatozoa of lead acid battery factory workers. Blood and semen samples were collected from total 80 workers (7-15 years exposure) and 40 non-occupationally exposed control subjects. The lead exposed battery factory workers showed lowering (P < 0.001) of sperm count, density, motility and semen volume along with an increase incidence of sperm abnormality and prolong liquefaction time. Structural alteration of sperm cell was prevalent among the exposed population as evidenced by significantly (P < 0.001) low sperm viability, low hypoosmotic swelling test (HOST) percentage, high lipid peroxidation of sperm membrane with concomitant alterations of seminal plasma total and dehydro ascorbate level. Sharp depressions, membrane folding and granularity at sperm head surfaces were observed by scanning electron microscopy (SEM). Both blood lead and semen lead was significantly (P < 0.001) higher among the factory workers. Thus it appears plausible that lead may reduce the antioxidant level in seminal plasma and enhance the lipid peroxidative changes in sperm membrane leading to concomitant structural damage of sperm cell surface in the workers employed in lead acid battery factories.