The FK506 binding protein Fpr3 counteracts protein phosphatase 1 to maintain meiotic recombination checkpoint activity

Cell. 2005 Sep 23;122(6):861-73. doi: 10.1016/j.cell.2005.07.010.


The meiotic recombination checkpoint delays gamete precursors in G2 until DNA breaks created during recombination are repaired and chromosome structure has been restored. Here, we show that the FK506 binding protein Fpr3 prevents premature adaptation to damage and thus serves to maintain recombination checkpoint activity. Impaired checkpoint function is observed both in cells lacking FPR3 and in cells treated with rapamycin, a small molecule inhibitor that binds to the proline isomerase (PPIase) domain of Fpr3. FPR3 functions in the checkpoint through controlling protein phosphatase 1 (PP1). Fpr3 interacts with PP1 through its PPIase domain, regulates PP1 localization, and counteracts the activity of PP1 in vivo. Our findings define a branch of the recombination checkpoint involved in the adaptation to persistent chromosomal damage and a critical function for FK506 binding proteins during meiosis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA Damage
  • Gene Expression Regulation
  • Immunophilins / genetics
  • Immunophilins / pharmacology
  • Immunophilins / physiology*
  • Meiosis*
  • Peptidylprolyl Isomerase / antagonists & inhibitors
  • Peptidylprolyl Isomerase / genetics
  • Phosphoprotein Phosphatases / antagonists & inhibitors*
  • Phosphoprotein Phosphatases / genetics
  • Phosphoprotein Phosphatases / physiology*
  • Point Mutation
  • Protein Phosphatase 1
  • Recombination, Genetic / physiology*
  • Saccharomyces cerevisiae / cytology
  • Saccharomyces cerevisiae / physiology
  • Saccharomyces cerevisiae Proteins / antagonists & inhibitors
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / pharmacology
  • Saccharomyces cerevisiae Proteins / physiology*
  • Sirolimus / pharmacology
  • Time Factors


  • Saccharomyces cerevisiae Proteins
  • GLC7 protein, S cerevisiae
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 1
  • FPR3 protein, S cerevisiae
  • Immunophilins
  • Peptidylprolyl Isomerase
  • Sirolimus