Characterization of Mesenchymal Stem Cells Isolated From Mouse Fetal Bone Marrow

Stem Cells. 2006 Mar;24(3):482-93. doi: 10.1634/stemcells.2005-0219. Epub 2005 Sep 22.

Abstract

Mesenchymal stem cells (MSCs) are defined as cells that can differentiate into multiple mesenchymal lineage cells. MSCs have some features (surface molecules and cytokine production, etc.) common to so-called traditional bone marrow (BM) stromal cells, which have the capacity to support hemopoiesis. In the present study, we isolated murine MSCs (mMSCs) from the fetal BM using an anti-PA6 monoclonal antibody (mAb) that is specific for bone marrow stromal cells. The mMSCs, called FMS/PA6-P cells, are adherent, fibroblastic, and extensively expanded and have the ability to differentiate not only into osteoblasts and adipocytes but also into vascular endothelial cells. The FMS/PA6-P cells produce a broad spectrum of cytokines and growth factors closely related to hemopoiesis and show good hemopoiesis-supporting capacity both in vivo and in vitro, suggesting that they are a component of the hemopoietic stem cell niche in vivo. Interestingly, although the FMS/PA6-P cells express a high level of the PA6 molecule, which is reactive with anti-PA6 mAb, they gradually lose their ability to express this molecule during the course of differentiation into osteoblasts and adipocytes, indicating that the PA6 molecule might serve as a novel marker of mMSCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology
  • Adipocytes / physiology
  • Animals
  • Antigens, Differentiation / biosynthesis
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / physiology*
  • Cell Differentiation / physiology*
  • Cells, Cultured
  • Female
  • Fetus / cytology
  • Fetus / physiology*
  • Growth Substances / biosynthesis
  • Hematopoiesis / physiology*
  • Male
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / physiology*
  • Mice
  • Mice, Nude
  • Osteoblasts / cytology
  • Osteoblasts / physiology
  • Stromal Cells / cytology
  • Stromal Cells / physiology

Substances

  • Antigens, Differentiation
  • Growth Substances