Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma

Leuk Res. 2006 Apr;30(4):449-57. doi: 10.1016/j.leukres.2005.08.016. Epub 2005 Sep 23.


The objectives of this study were foremost to further characterize pre-existing cell lines containing the t(11;14)(q13;q32) translocation. This translocation along with cyclin D1 overexpression is characteristic of Mantle Cell Lymphoma (MCL), an aggressive B cell neoplasm. Considerable variation in the abundance of cyclin D1 expression was observed. mRNA levels were examined by RT-PCR as differences in cyclin D1 mRNA abundance have been shown to synergize with INK4A/Arf deletions to dictate proliferation rate and survival in MCL patient samples. In this study, the cell lines, Z-138 and HBL-2, which exhibited the fastest growth rates and the shortest survival times in Rag2-M mice, had high expression of either one or both cyclin D1 mRNA isoforms and had negligible expression of p16. On the other hand, NCEB-1 and JVM-2 had low expression of both mRNA isoforms, retained p16 expression, and had slower growth rates and exhibited longer survival times in Rag2-M mice. Furthermore, JVM-2, which was found to have the lowest expression of cyclin D1, was the only cell line that expressed cyclin D2. The results of the characterization of Z-138, HBL-2, NCEB-1 and JVM-2 reveal that this group of cell lines represents both classic and variant features of MCL.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Western
  • Cell Line, Tumor
  • Chromosomes, Human, Pair 11*
  • Cyclin D1 / genetics
  • DNA Primers
  • Female
  • Herpesvirus 4, Human / isolation & purification
  • Humans
  • Immunophenotyping
  • In Situ Hybridization, Fluorescence
  • Karyotyping
  • Lymphoma, Mantle-Cell / genetics*
  • Lymphoma, Mantle-Cell / pathology
  • Lymphoma, Mantle-Cell / virology
  • Male
  • Mice
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • Translocation, Genetic*


  • DNA Primers
  • RNA, Messenger
  • Cyclin D1