Role of fascin in the proliferation and invasiveness of esophageal carcinoma cells

Biochem Biophys Res Commun. 2005 Nov 11;337(1):355-62. doi: 10.1016/j.bbrc.2005.09.055.


Fascin, an actin-bundling protein, induces membrane protrusions and increases cell motility in various transformed cells. The overexpression of fascin in esophageal squamous cell carcinoma (ESCC) has been described only recently, but the roles and mechanism still remained unclear. Here, by using RNA interference (RNAi), we have stably silenced the expression of the fascin in EC109 cells, an ESCC cell line. Down-regulation of fascin resulted in a suppression of cell proliferation and as well as a decrease in cell invasiveness. Furthermore, we revealed that fascin might have functions in regulating tumor growth in vivo. The effect of fascin on cell invasiveness correlated with the activation of matrix metalloproteases such as MMP-2 and MMP-9. We examined that fascin down-expression also led to a decrease of c-erbB-2 and beta-catenin at the protein level. These results suggested that fascin might play crucial roles in regulating neoplasm progression of ESCC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma, Squamous Cell / metabolism*
  • Carcinoma, Squamous Cell / pathology*
  • Carrier Proteins / antagonists & inhibitors
  • Carrier Proteins / genetics
  • Carrier Proteins / physiology*
  • Cell Line, Tumor
  • Cell Proliferation
  • Cytoskeletal Proteins / metabolism
  • Down-Regulation
  • Esophageal Neoplasms / metabolism*
  • Esophageal Neoplasms / pathology*
  • HeLa Cells
  • Humans
  • Mice
  • Mice, Nude
  • Microfilament Proteins / antagonists & inhibitors
  • Microfilament Proteins / genetics
  • Microfilament Proteins / physiology*
  • Neoplasm Invasiveness
  • RNA Interference
  • Receptor, ErbB-2 / metabolism
  • Trans-Activators / metabolism
  • beta Catenin


  • CTNNB1 protein, human
  • CTNNB1 protein, mouse
  • Carrier Proteins
  • Cytoskeletal Proteins
  • Microfilament Proteins
  • Trans-Activators
  • beta Catenin
  • fascin
  • Receptor, ErbB-2