Expressions and clinical significances of angiopoietin-1, -2 and Tie2 in human gastric cancer

Biochem Biophys Res Commun. 2005 Nov 11;337(1):386-93. doi: 10.1016/j.bbrc.2005.09.051.


The roles of angiopoietins in gastric cancer progression are still not fully understood. In this study the expressions of angiopoietin-1 (Ang-1), -2 (Ang-2) were compared by immunohistochemistry in 53 gastric cancer and 23 normal gastric mucosa samples. Results revealed that Ang-2 expression was significantly increased in gastric cancer tissues (74%) and was correlated with higher TNM stage, lymph node metastasis as well as distance metastasis. The expression of Ang-1 was also elevated in cancerous tissues (66%) and significantly associated with differentiation degree. In addition, Ang-2 as well as its receptor Tie2 expressions were higher in 12 pairs of gastric cancer tissue samples than those in corresponding adjacent samples by Western blot, while Ang-1 expression showed great heterogeneity. Furthermore, the expressions of Ang-1 and Ang-2 were almost positive in eight gastric cancer cell lines. Among them, AGS expressed both Ang-2 and a relatively moderate amount of Ang-2(443), a novel splice form of Ang-2, while others showed only Ang-2 mRNA expression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiopoietin-1 / genetics
  • Angiopoietin-1 / metabolism*
  • Angiopoietin-2 / genetics
  • Angiopoietin-2 / metabolism*
  • Blotting, Western
  • Female
  • Gastric Mucosa / metabolism
  • Humans
  • Immunohistochemistry
  • Male
  • RNA, Messenger / metabolism
  • Receptor, TIE-2 / genetics
  • Receptor, TIE-2 / metabolism*
  • Stomach Neoplasms / diagnosis*
  • Stomach Neoplasms / metabolism
  • Stomach Neoplasms / pathology


  • Angiopoietin-1
  • Angiopoietin-2
  • RNA, Messenger
  • Receptor, TIE-2