Photoregulation of DNA polymerase I (Klenow) with caged fluorescent oligodeoxynucleotides

Bioorg Med Chem Lett. 2005 Dec 1;15(23):5303-6. doi: 10.1016/j.bmcl.2005.08.058. Epub 2005 Sep 26.


The DNA polymerase reaction by Klenow fragment (KF) was efficiently regulated with UV light using a 25-mer caged fluorescent oligodeoxynucleotide (CFO) as the template. The CFO was functionalized with a fluorescein reporter (Fl) and photocleavable DABSYL quencher moiety (Dab). With Fl and Dab at adjacent cytidines in the middle at the template, KF was blocked from extending a complementary 12-mer primer. Upon UV photolysis of the DABSYL blocking group under aerobic conditions, fluorescein emission was restored and 50% of the primers were fully extended by KF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Polymerase I / metabolism*
  • Fluorescent Dyes / chemistry*
  • Oligodeoxyribonucleotides / chemistry*
  • Oligodeoxyribonucleotides / metabolism
  • Oligodeoxyribonucleotides / radiation effects*
  • Photolysis
  • Templates, Genetic
  • Ultraviolet Rays*


  • Fluorescent Dyes
  • Oligodeoxyribonucleotides
  • DNA Polymerase I