Developmental regulation of protein kinase B activation is isoform specific in skeletal muscle of neonatal pigs

Pediatr Res. 2005 Oct;58(4):719-24. doi: 10.1203/01.PDR.0000180536.51032.AB.


The postprandial activation of the insulin signaling pathway that leads to translation initiation is enhanced in skeletal muscle of the neonate and decreases with development in parallel with the developmental decline in muscle protein synthesis. Our previous study showed that the activity of protein kinase B (PKB), a major insulin signaling component, was higher in 7- than in 26-d-old pigs. To examine the molecular mechanisms involved, we determined PKB isoform abundance and phosphorylation state, the abundance of its kinases, and PKB's association with its kinases. The abundances of total PKB, PKBalpha, and PKBgamma were higher in muscle of 7- than in 26-d-old pigs whereas PKBbeta abundance was similar in the two age groups. PKB phosphorylation at Thr308 was higher in 7- than in 26-d-old pigs but PKB phosphorylation at Ser473 was similar in both age groups. The association of PKB with 3'-phosphoinositide-dependent kinase-1 (PDK-1), a kinase that phosphorylates PKB at Thr308, and PDK-1 abundance were higher in 7- than in 26-d-old pigs. Moreover, PDK-1 phosphorylation at Ser-241, a site that is crucial for PDK-1 activation, was higher in 7- than in 26-d-old pigs. However, the association of PKB with integrin-linked kinase (ILK), a kinase that potentially phosphorylates PKB at Ser473, and ILK abundance were similar in both age groups. The result suggests that the developmental change in PKB activation is isoform specific and involves regulation by PDK-1.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3-Phosphoinositide-Dependent Protein Kinases
  • Animals
  • Animals, Newborn
  • Blotting, Western
  • Gene Expression Regulation, Developmental*
  • Gene Expression Regulation, Enzymologic*
  • Immunoprecipitation
  • Insulin / metabolism
  • Muscle, Skeletal / embryology*
  • Muscle, Skeletal / enzymology
  • Muscle, Skeletal / metabolism
  • Phosphorylation
  • Protein Isoforms
  • Protein Serine-Threonine Kinases / metabolism
  • Proto-Oncogene Proteins c-akt / chemistry*
  • Proto-Oncogene Proteins c-akt / metabolism*
  • Serine / chemistry
  • Signal Transduction
  • Swine
  • Threonine / chemistry
  • Time Factors


  • Insulin
  • Protein Isoforms
  • Threonine
  • Serine
  • integrin-linked kinase
  • 3-Phosphoinositide-Dependent Protein Kinases
  • Protein Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-akt