Culture-independent analysis of fecal microbiota in cattle

Biosci Biotechnol Biochem. 2005 Sep;69(9):1793-7. doi: 10.1271/bbb.69.1793.


The phylogenetic diversity of the fecal bacterial community in Holstein cattle was determined by 16S ribosomal RNA gene sequence analysis. The sequences were affiliated with the following phyla: Firmicutes (81.3%), Bacteroidetes (14.4%), Actinobacteria (2.5%), and Proteobacteria (1.4%). The Clostridium leptum subgroup was the most phylogenetically diverse group in cattle feces. In addition, a number of previously uncharacterized and unidentified bacteria were recognized in clone libraries.

MeSH terms

  • Animals
  • Bacteria / classification*
  • Cattle
  • Feces / microbiology*
  • Genes, rRNA*
  • Phylogeny
  • Sequence Analysis / methods*