Spectral domain second-harmonic optical coherence tomography

Opt Lett. 2005 Sep 15;30(18):2391-3. doi: 10.1364/ol.30.002391.


Optical coherence tomography (OCT) provides micrometer-scale structural imaging by coherent detection of backscattered light. Molecular contrast in OCT has been demonstrated using transient absorption, coherent anti-Stokes Raman scattering, and second-harmonic (SH) generation. The sensitivity of molecular contrast signals can be enhanced by use of Fourier domain techniques. We have constructed a spectrometer-based Fourier domain SH-OCT system for simultaneous acquisition of the fundamental and SH signals. We report a >30 dB increase in SH sensitivity over a similar time domain SH-OCT system and demonstrate contrast between cartilage and bone using collagen as the contrast agent.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Equipment Design
  • Equipment Failure Analysis
  • Image Enhancement / instrumentation*
  • Image Enhancement / methods
  • Microscopy, Interference / instrumentation*
  • Microscopy, Interference / methods
  • Spectroscopy, Fourier Transform Infrared / instrumentation*
  • Spectroscopy, Fourier Transform Infrared / methods
  • Tomography, Optical Coherence / instrumentation*
  • Tomography, Optical Coherence / methods