Cytotoxic and proliferative lymphocyte responses to allogeneic and xenogeneic antigens in vitro

Aust J Exp Biol Med Sci. 1979 Oct;57(5):467-77. doi: 10.1038/icb.1979.48.


In vitro lymphoproliferative responses to foreign histocompatibility antigens are phylogenetically restricted. Responses occur most readily to allogeneic or closely related xenogeneic leucocytes, but not to unrelated xenogeneic cells. Specific cytotoxic T cell responses to foreign histocompatibility antigens show the same phylogenetic restriction. This lack of xenoreactivity is not due to a lack of precursor cells for the xenoantigens; guinea-pig lymphocytes, although normally unresponsive to mouse antigens, have a similar precursor frequency for these antigens as do lymphocytes of allogeneic mouse strains. Specific cytotoxic responses of guinea-pig lymphocytes to mouse antigens can be generated if a factor released from con A stimulated guinea-pig spleen cells is added to the culture medium. The factor produced by con A-activated spleen cells (CS) is also phylogenetically restricted in its action; CS must be obtained from animals homologous with the donor of the responding lymphocytes.

MeSH terms

  • Animals
  • Antigens, Heterophile / immunology*
  • Cobalt Radioisotopes
  • Cytotoxicity Tests, Immunologic
  • Goats
  • Guinea Pigs
  • Histocompatibility Antigens / immunology*
  • In Vitro Techniques
  • Isoantigens / immunology*
  • Leukocytes / immunology*
  • Lymph Nodes / cytology
  • Lymphocyte Culture Test, Mixed
  • Lymphocytes / immunology*
  • Male
  • Mice
  • Phylogeny
  • Rats
  • Sheep
  • Spleen / cytology


  • Antigens, Heterophile
  • Cobalt Radioisotopes
  • Histocompatibility Antigens
  • Isoantigens