Transcriptional repression by a novel member of the bZIP family of transcription factors

Mol Cell Biol. 1992 Jul;12(7):3070-7. doi: 10.1128/mcb.12.7.3070-3077.1992.


We describe here a novel member of the bZIP family of DNA-binding proteins, designated E4BP4, that displays an unusual DNA-binding specificity which overlaps that of the activating transcription factor family of factors. When expressed in a transient transfection assay with a suitable reporter plasmid, E4BP4 strongly repressed transcription in a DNA-binding-site-dependent manner. Examination of a series of deletion mutants revealed that sequences responsible for the repressing potential of E4BP4 lie within the carboxyl-terminal region of the protein. No similarity was found between this region and the repressing domains of other known eukaryotic transcriptional repressors.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Basic-Leucine Zipper Transcription Factors
  • Binding Sites
  • DNA Mutational Analysis
  • DNA-Binding Proteins / genetics*
  • G-Box Binding Factors
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Placenta
  • Plant Proteins / genetics*
  • Protein Conformation
  • Repressor Proteins / genetics*
  • Sequence Homology, Nucleic Acid
  • Transcription Factors*
  • Transcription, Genetic
  • Transfection


  • Basic-Leucine Zipper Transcription Factors
  • DNA-Binding Proteins
  • G-Box Binding Factors
  • NFIL3 protein, human
  • Plant Proteins
  • Repressor Proteins
  • Transcription Factors

Associated data

  • GENBANK/X65318