FAK and PYK2 interact with SAP90/PSD-95-Associated Protein-3

Biochem Biophys Res Commun. 2005 Nov 18;337(2):641-6. doi: 10.1016/j.bbrc.2005.09.099. Epub 2005 Sep 26.


Focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2) are two related non-receptor tyrosine kinases highly expressed in brain. Although they are both involved in synaptic plasticity, little is known about their specific neuronal partners. Using a yeast two-hybrid screen and GST pull-down assays we show that SAPAP3 (SAP90/PSD-95-Associated Protein-3) interacts with FAK (residues 676-840) and PYK2. The three proteins partly co-distribute in the same sucrose gradient fractions as the post-synaptic density protein PSD-95 and Src. Our results suggest that SAPAP3 is an anchoring protein for FAK and PYK2 in post-synaptic densities and may contribute to the synaptic function of these tyrosine kinases.

MeSH terms

  • Animals
  • Brain / enzymology*
  • COS Cells
  • Centrifugation, Density Gradient
  • Chlorocebus aethiops
  • Enzyme Activation
  • Focal Adhesion Kinase 2 / metabolism*
  • Focal Adhesion Protein-Tyrosine Kinases / metabolism*
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / metabolism*
  • Phosphorylation
  • SAP90-PSD95 Associated Proteins
  • Subcellular Fractions
  • Synapses / chemistry
  • Synapses / metabolism*
  • Synaptosomes / metabolism
  • Two-Hybrid System Techniques
  • Tyrosine / chemistry
  • Tyrosine / metabolism


  • Nerve Tissue Proteins
  • SAP90-PSD95 Associated Proteins
  • Tyrosine
  • Focal Adhesion Kinase 2
  • Focal Adhesion Protein-Tyrosine Kinases