Small ubiquitin-like modifier (SUMO) recognition of a SUMO binding motif: a reversal of the bound orientation

J Biol Chem. 2005 Dec 2;280(48):40122-9. doi: 10.1074/jbc.M507059200. Epub 2005 Oct 3.

Abstract

Sumoylation has recently been identified as an important mechanism that regulates protein interactions and localization in essential cellular functions, such as gene transcription, subnuclear structure formation, viral infection, and cell cycle progression. A SUMO binding amino acid sequence motif (SBM), which recognizes the SUMO moiety of modified proteins in sumoylation-dependent cellular functions, has been consistently identified by several recent studies. To understand the mechanism of SUMO recognition by the SBM, we have solved the solution structure of SUMO-1 in complex with a peptide containing the SBM derived from the protein PIASX (KVDVIDLTIESSSDEEEDPPAKR). Surprisingly, the structure reveals that the bound orientation of the SBM can reverse depending on the sequence context. The structure also reveals a novel mechanism of recognizing target sequences by a ubiquitin-like module. Unlike ubiquitin binding motifs, which all form helices and bind to the main beta-sheet of ubiquitin, the SBM forms an extended structure that binds between the alpha-helix and a beta-strand of SUMO-1. This study provides a clear mechanism of the SBM sequence variations and its recognition of the SUMO moiety in sumoylated proteins.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Calorimetry
  • Cell Cycle
  • Humans
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • Molecular Sequence Data
  • Mutation
  • Peptides / chemistry
  • Protein Binding
  • Protein Conformation
  • Protein Inhibitors of Activated STAT / chemistry
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Small Ubiquitin-Related Modifier Proteins / chemistry*
  • Software
  • Transcription, Genetic
  • Ubiquitin / chemistry

Substances

  • PIAS2 protein, human
  • Peptides
  • Protein Inhibitors of Activated STAT
  • Small Ubiquitin-Related Modifier Proteins
  • Ubiquitin

Associated data

  • PDB/2ASQ