Evaluation of universal probes and primer sets for assessing total bacterial load in clinical samples: general implications and practical use in endodontic antimicrobial therapy

J Clin Microbiol. 2005 Oct;43(10):5332-7. doi: 10.1128/JCM.43.10.5332-5337.2005.

Abstract

By re-examining 10 previously published "universal" PCR assays using the ARB phylogenetic software package and database with 41,000 16S rRNA gene sequences, we found that they differed considerably in their coverage of the domain Bacteria. We evaluated the broadest-range real-time quantitative PCR protocol for its efficacy in measuring the antimicrobial effects of endodontic treatments.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Anti-Infective Agents, Local / administration & dosage
  • Anti-Infective Agents, Local / therapeutic use*
  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / isolation & purification*
  • Bacterial Infections / drug therapy
  • Bacterial Infections / microbiology
  • Chlorhexidine / administration & dosage
  • Chlorhexidine / therapeutic use*
  • DNA Probes*
  • Dental Pulp Cavity / microbiology
  • Endodontics / methods
  • Humans
  • Middle Aged
  • Periapical Diseases / drug therapy
  • Periapical Diseases / microbiology*
  • Phylogeny
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 16S / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sodium Hypochlorite / administration & dosage
  • Sodium Hypochlorite / therapeutic use*
  • Software

Substances

  • Anti-Infective Agents, Local
  • DNA Probes
  • RNA, Ribosomal, 16S
  • Sodium Hypochlorite
  • Chlorhexidine