Changes in immune responses to antigen applied to tape-stripped skin with CpG-oligodeoxynucleotide in mice

J Control Release. 2005 Nov 28;108(2-3):294-305. doi: 10.1016/j.jconrel.2005.08.014. Epub 2005 Oct 4.


CpG-oligodeoxynucleotide (CpG-ODN) plays a critical role in immunity via the augmentation of Th1 and suppression of Th2 responses. We examined here the effect of CpG-ODN on the immune response to an antigen applied to tape-stripped mouse skin by evaluating the production of cytokines and Ig isotypes. Confocal laser scanning microscopy revealed that the model antigen, OVA, and CpG-ODN easily penetrated the tape-stripped skin. Co-administration of CpG-ODN and OVA to the disrupted skin elicited an antigen-specific Th1-predominant immune response and enhanced the production of Th1-type cytokines, IL-12 and IFN-gamma. On the other hand, the production of a Th2-type cytokine, IL-4, was drastically suppressed. Cytokine production was supported by the expression of mRNA in the draining lymph node. In terms of antigen-specific antibody production, the level of IgG2a which is regulated by IFN-gamma was increased by CpG-ODN, but IgE production regulated by IL-4 was suppressed. Furthermore, administration of CpG-ODN via the skin drastically attenuated the production of IgE in mice undergoing IgE-type immune response. Administration of CpG-ODN through the skin may shift the immune response from Th2 to Th1-like response. These results suggested that administration of CpG-ODN via skin is a simple strategy for patients with diseases like AD, which is characterized by Th2-dominated inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic*
  • Administration, Topical
  • Animals
  • Antibodies / analysis
  • Antibody Specificity
  • Antigens / administration & dosage*
  • Antigens / immunology*
  • Cytokines / metabolism
  • Dermatitis, Atopic / immunology
  • Dermatitis, Atopic / therapy
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Oligonucleotides / administration & dosage
  • Oligonucleotides / pharmacology*
  • Ovalbumin / immunology
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Skin Absorption / physiology*
  • Spleen / cytology
  • Spleen / immunology
  • Th1 Cells / immunology
  • Th2 Cells / immunology


  • Adjuvants, Immunologic
  • Antibodies
  • Antigens
  • Cytokines
  • Oligonucleotides
  • RNA, Messenger
  • Ovalbumin