Reproductive aging results in a reconfigured ovarian antioxidant defense profile in rats

Fertil Steril. 2005 Oct:84 Suppl 2:1109-13. doi: 10.1016/j.fertnstert.2005.02.054.


Objective: To test our hypothesis that reproductive aging changes the ovarian oxidative stress defense profile, in response to prostaglandin F2alpha (PGF2alpha) during corpus luteum regression, because how a cell or an organ handles reactive oxygen intermediates may be dependent on the biological age of the organism.

Design: Animal experimentation using rat model of corpus luteum regression.

Setting: University reproductive biology laboratory.

Animal(s): Control (26-day-old) and 8- to 9-month-old (reproductive aging) rats.

Intervention(s): Corpus luteum formation was induced in control and 8- to 9-month-old (reproductive aging) rats with pregnant mare serum gonadotropin followed by human chorionic gonadotropin. Regression was then initiated with PGF2alpha.

Main outcome measure(s): Vitamin E, glutathione reductase, glutathione peroxidase, catalase, and thiobarbituric acid-reacting substances were measured.

Result(s): Ovaries from reproductive aging rats, compared with the control (26-day-old) group, had elevated vitamin E levels at 0, 2, and 24 hours after PGF2alpha. At 2 and 24 hours after PGF2alpha, the aging ovaries had lower glutathione reductase levels.

Conclusion(s): These data suggest that the reproductive aging ovary has a transformed oxidative stress defense profile and that this may account for some of the physiological changes found in reproductive aging.

Publication types

  • Comparative Study

MeSH terms

  • Aging / metabolism*
  • Animals
  • Antioxidants / metabolism*
  • Female
  • Ovary / metabolism*
  • Oxidative Stress / physiology
  • Rats
  • Rats, Sprague-Dawley
  • Reproduction / physiology*


  • Antioxidants