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. Sep-Oct 2005;4(5):1752-6.
doi: 10.1021/pr050225e.

I-DIRT, a General Method for Distinguishing Between Specific and Nonspecific Protein Interactions

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I-DIRT, a General Method for Distinguishing Between Specific and Nonspecific Protein Interactions

Alan J Tackett et al. J Proteome Res. .

Abstract

Isolation of protein complexes via affinity-tagged proteins provides a powerful tool for studying biological systems, but the technique is often compromised by co-enrichment of nonspecifically interacting proteins. We describe a new technique (I-DIRT) that distinguishes contaminants from bona fide interactors in immunopurifications, overcoming this most challenging problem in defining protein complexes. I-DIRT will be of broad value for studying protein complexes in biological systems that can be metabolically labeled.

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